小鼠α白细胞干扰素基因的分离及细菌表达
Isolation and bacterial expression of a murine alpha leukocyte interferon gene.
作者信息
Daugherty B, Martin-Zanca D, Kelder B, Collier K, Seamans T C, Hotta K, Pestka S
出版信息
J Interferon Res. 1984 Fall;4(4):635-43. doi: 10.1089/jir.1984.4.635.
PMID:6094686
Abstract
A murine alpha interferon gene (MuIFN-alpha A) was isolated from a murine genomic DNA library in phage lambda. The gene was cloned and its sequence determined. It was expressed in Escherichia coli under the control of the trp promoter/operator which resulted in antiviral activity on mouse L-cells. MuIFN-alpha A exhibits 60% homology to HuIFN-alpha A at the amino acid level.
摘要
从小鼠基因组DNA噬菌体λ文库中分离出一个小鼠α干扰素基因（MuIFN-αA）。该基因被克隆并测定了其序列。它在色氨酸启动子/操纵子的控制下在大肠杆菌中表达，从而对小鼠L细胞产生抗病毒活性。MuIFN-αA在氨基酸水平上与HuIFN-αA具有60%的同源性。
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