一种功能性小鼠干扰素α基因的分离与鉴定，该基因在病毒诱导下不在成纤维细胞中表达。

Isolation and characterization of a functional murine interferon alpha gene which is not expressed in fibroblasts upon virus induction.

作者信息

Navarro S, Dion M, Vodjdani G, Berlot-Picard F, Doly J

机构信息

Laboratoire de Biologie Moléculaire des Interférons, UPR 37 CNRS, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.

出版信息

J Gen Virol. 1989 Jun;70 ( Pt 6):1381-9. doi: 10.1099/0022-1317-70-6-1381.

DOI:10.1099/0022-1317-70-6-1381

PMID:2471809

Abstract

A mouse genomic segment containing three new members of the murine interferon alpha (MuIFN-alpha) gene family was isolated from a fibroblastic cosmid library. A 4 kb EcoRI fragment contained a new MuIFN-alpha gene named MuIFN-alpha 8. The nucleotide sequence of the coding and flanking regions of this gene showed a high level of homology to those of known members of the MuIFN-alpha family. Transient expression of the MuIFN-alpha 8 gene in COS cells and oocyte translation of in vitro transcripts both led to a biologically active protein. The antiviral activity was neutralized by monoclonal and polyclonal MuIFN-alpha antibodies. Although the 5' flanking sequence shows features characteristic of an IFN regulatory region, the MuIFN-alpha 8 gene is not expressed in murine fibroblasts treated with Newcastle disease virus or poly(I).poly(C).

摘要

从成纤维细胞黏粒文库中分离出一个包含鼠干扰素α（MuIFN-α）基因家族三个新成员的小鼠基因组片段。一个4kb的EcoRI片段包含一个名为MuIFN-α 8的新MuIFN-α基因。该基因编码区和侧翼区的核苷酸序列与MuIFN-α家族已知成员的序列具有高度同源性。MuIFN-α 8基因在COS细胞中的瞬时表达以及体外转录本在卵母细胞中的翻译均产生了具有生物活性的蛋白质。抗病毒活性被单克隆和多克隆MuIFN-α抗体中和。尽管5'侧翼序列显示出IFN调节区的特征性特点，但MuIFN-α 8基因在用新城疫病毒或聚肌苷酸:聚胞苷酸（poly(I).poly(C)）处理的小鼠成纤维细胞中不表达。