Farrar G H, Oram J D
J Gen Virol. 1984 Nov;65 ( Pt 11):1991-2001. doi: 10.1099/0022-1317-65-11-1991.
Virions of human cytomegalovirus were shown to contain two discrete membranes. An outer, loose fitting, membrane was sensitive to osmotic shock and could be partially removed by diluting buffered preparations of virions with water. Purified virions were shown, by SDS-polyacrylamide gel electrophoresis of virion membranes labelled by carbohydrate-specific procedures, to contain five glycoproteins with molecular weights of 52, 67, 95, 130 and 250, all X 10(3). Digestion of virions with endoglycosidases revealed that there were structural differences between the carbohydrate portions of the glycoproteins. All five glycoproteins were recognized by antibodies present in pools of human convalescent sera.
已证明人类巨细胞病毒的病毒粒子含有两层离散的膜。外层的、贴合较松的膜对渗透压休克敏感，用水稀释病毒粒子的缓冲制剂时可部分去除。通过对经碳水化合物特异性方法标记的病毒粒子膜进行SDS-聚丙烯酰胺凝胶电泳显示，纯化的病毒粒子含有五种糖蛋白，分子量分别为52、67、95、130和250，均为X 10(3)。用内切糖苷酶消化病毒粒子表明，这些糖蛋白的碳水化合物部分存在结构差异。所有这五种糖蛋白都能被人类恢复期血清池中的抗体识别。
