单纯疱疹病毒与阿尔茨海默病：通过斑点杂交法寻找病毒DNA
Herpes simplex virus and Alzheimer's disease: a search for virus DNA by spot hybridisation.
作者信息
Taylor G R, Crow T J, Markakis D A, Lofthouse R, Neeley S, Carter G I
出版信息
J Neurol Neurosurg Psychiatry. 1984 Oct;47(10):1061-5. doi: 10.1136/jnnp.47.10.1061.
A sensitive hybridisation screening technique (spot hybridisation) was used to search post-mortem brain DNA extracts for herpes simplex I virus sequences in non-neurological controls, Alzheimer's disease cases and Herpes simplex virus infected mice. A reconstruction experiment showed that 0.1 herpes genome equivalents per cell could be detected in 100 micrograms tissue extracts. Although herpes sequences were readily detected in infected mice, none of the human brains examined could be shown to contain such sequences.
一种灵敏的杂交筛选技术（斑点杂交）被用于在非神经学对照、阿尔茨海默病病例以及单纯疱疹病毒感染小鼠的尸检脑DNA提取物中搜寻单纯疱疹病毒I型序列。一项重建实验表明，在100微克组织提取物中每细胞可检测到0.1个疱疹基因组当量。尽管在感染小鼠中很容易检测到疱疹序列，但所检测的人脑均未显示含有此类序列。
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