O'Donohue T L, Massari V J, Pazoles C J, Chronwall B M, Shults C W, Quirion R, Chase T N, Moody T W
J Neurosci. 1984 Dec;4(12):2956-62. doi: 10.1523/JNEUROSCI.04-12-02956.1984.
Bombesin (BN)-containing neuronal processes were demonstrated in laminae I and II of the dorsal horn of the cat, rat, and mouse spinal cord by immunocytochemistry and radioimmunoassay. Dorsal rhizotomy in the cat resulted in a marked decrease in BN immunoreactivity in the dorsal horn indicating that BN is contained in primary sensory afferents. BN-binding sites were also localized in superficial laminae of the dorsal horn. The presence of both BN and BN-binding sites in the dorsal horn suggested that BN may be involved in sensory processing in the spinal cord. Consistent with this hypothesis, it was demonstrated that an injection of BN into the spinal cord caused a biting and scratching response indicative of sensory stimulation. The effect was similar to that observed after injection of substance P into the cord with the exception that the BN effect lasted about 100 times longer than that induced by substance P. Taken together, these data indicate that BN may be a neurotransmitter of primary sensory afferents to the spinal cord.
通过免疫细胞化学和放射免疫分析在猫、大鼠和小鼠脊髓背角的Ⅰ层和Ⅱ层中证实了含有蛙皮素（BN）的神经突。猫的背根切断术导致背角中BN免疫反应性显著降低，表明BN存在于初级感觉传入纤维中。BN结合位点也定位于背角的浅层。背角中BN和BN结合位点的存在表明BN可能参与脊髓的感觉处理。与该假设一致的是，已证明将BN注入脊髓会引起咬和抓挠反应，这表明存在感觉刺激。该效应与向脊髓注射P物质后观察到的效应相似，不同之处在于BN的效应持续时间比P物质诱导的效应长约100倍。综上所述，这些数据表明BN可能是脊髓初级感觉传入纤维的一种神经递质。
