Panula P, Yang H Y, Costa E
Regul Pept. 1982 Oct;4(5):275-83. doi: 10.1016/0167-0115(82)90120-3.
The immunohistochemical distribution of bombesin-like immunoreactivity in the central nervous system of the rat was revealed using a rabbit antibody against [Glu7]bombesin(6-14). In radioimmunoassay, the antibody had minimal cross reactivity with substance P thereby enhancing the significance of histochemical controls proving that the immunoreactivity detected was related to bombesin but not to substance P. Bombesin-immunoreactive neurons were detected in several brain structures including the hypothalamus, interpeduncular nucleus, central grey, dorsolateral tegmental nucleus, dorsal parabrachial nucleus, nucleus of the solitary tract and trigeminal complex. In the spinal cord, intense immunoreactivity was found in the superficial layers of the posterior horn. Since in this area the reaction diminished after rhizotomy the location of the peptide in afferent neurons was considered. In the anterior horn the bombesin-like immunoreactivity located in nerve terminal-like structures was unchanged after rhizotomy suggesting that the cell bodies were located in CNS.
使用抗[Glu7]蛙皮素（6 - 14）的兔抗体，揭示了蛙皮素样免疫反应性在大鼠中枢神经系统中的免疫组织化学分布。在放射免疫分析中，该抗体与P物质的交叉反应极小，从而增强了组织化学对照的意义，证明检测到的免疫反应性与蛙皮素有关，而与P物质无关。在包括下丘脑、脚间核、中央灰质、背外侧被盖核、臂旁背核、孤束核和三叉神经复合体在内的几个脑结构中检测到了蛙皮素免疫反应性神经元。在脊髓中，在后角浅层发现了强烈的免疫反应性。由于在该区域神经根切断后反应减弱，因此考虑了肽在传入神经元中的定位。在前角，神经根切断后位于神经末梢样结构中的蛙皮素样免疫反应性没有变化，这表明细胞体位于中枢神经系统。
