Kleinberg M E, Finkelstein A
J Membr Biol. 1984;80(3):257-69. doi: 10.1007/BF01868444.
Nystatin forms two types of channels in sterol-containing planar bilayer membranes. One type is formed when it is added to only one side of the membrane; the other is formed when it is added to both sides of the membrane. The relative permeability of these channels to nonelectrolytes (urea and glycerol) is identical. The sensitivity of membranes to the one-sided action of nystatin is critically dependent on their thickness; in particular, membranes made from monoglycerides with more than 18 carbon atoms in their acyl chain are insensitive to nystatin's one-sided action. These data are consistent with a model in which the two types of channels formed by nystatin have essentially identical structures, except that the channel formed by its two-sided action is twice the length of that formed by its one-sided action, because it is a tail-to-tail dimer of the latter.
制霉菌素在含甾醇的平面双层膜中形成两种类型的通道。一种类型是在将其添加到膜的仅一侧时形成的；另一种类型是在将其添加到膜的两侧时形成的。这些通道对非电解质（尿素和甘油）的相对渗透率是相同的。膜对制霉菌素单侧作用的敏感性严重依赖于它们的厚度；特别是，由酰基链中碳原子数超过18个的甘油单酯制成的膜对制霉菌素的单侧作用不敏感。这些数据与一个模型一致，在该模型中，制霉菌素形成的两种类型的通道具有基本相同的结构，只是由其双侧作用形成的通道是由其单侧作用形成的通道长度的两倍，因为它是后者的尾对尾二聚体。
