Mechlinski W, Schaffner C P
J Antibiot (Tokyo). 1980 Jun;33(6):591-9. doi: 10.7164/antibiotics.33.591.
High performance liquid chromatographic (HPLC) procedures were utilized for the rapid and efficient separation and characterization of the aromatic heptaene macrolide group of antifungal antibiotics. The instrument utilized a 350 nm phosphor converted ultraviolet detector and a mu Bondapak C18 column packing. Optimum resolution of eleven commercial aromatic heptaene macrolide preparations was obtained with solvent systems consisting of mixtures of acetonitrile and 0.05 M aqueous sodium citrate buffer, pH 5.3. The presence of two distinct types of aromatic heptaene macrolides with numerous well-defined individual components has been established.
高效液相色谱（HPLC）方法被用于快速、高效地分离和鉴定抗真菌抗生素的芳香七烯大环内酯类。该仪器使用了一个350nm磷光转换紫外检测器和一个μ Bondapak C18柱填料。使用由乙腈和0.05M柠檬酸水溶液缓冲液（pH 5.3）的混合物组成的溶剂系统，获得了11种市售芳香七烯大环内酯制剂的最佳分离度。已确定存在两种不同类型的芳香七烯大环内酯，它们具有许多明确的单个成分。
