Rowe D T, Rowe M, Evan G I, Wallace L E, Farrell P J, Rickinson A B
EMBO J. 1986 Oct;5(10):2599-607. doi: 10.1002/j.1460-2075.1986.tb04540.x.
Certain newly established Epstein-Barr virus-containing Burkitt's lymphoma cell lines do not express the cytotoxic T-lymphocyte-detected membrane antigen (LYDMA) through which EBV infection is normally controlled by the host. When the EB virus recovered from these BL lines was used to transform peripheral blood lymphocytes from seronegative donors, the lymphoblastoid cell lines (LCLs) that arose were all LYDMA positive. This indicates that the LYDMA-negative nature of the BLs is not the result of a mutation in the resident viral genome but is rather a specific adaptation in those cells, perhaps permitting evasion of the host immune surveillance in tumour development. A comparison of the EBV gene expression in six LYDMA-negative and two LYDMA-positive BL lines and in their corresponding LCLs revealed that several of the BL lines did not express all of the viral gene products classically associated with latent transformation by EBV. Four out of eight cell lines showed restricted expression of the latent membrane protein (LMP) and/or the EB nuclear antigen, EBNA 2. A new level of EBV gene regulation therefore appears to be operating in some of the BL cell lines. The patterns of expression of EBV genes in the cell lines did not show any correlation with the known susceptibility of the lines to T cell killing.
某些新建立的含爱泼斯坦-巴尔病毒的伯基特淋巴瘤细胞系不表达细胞毒性T淋巴细胞检测到的膜抗原（LYDMA），而宿主通常通过该抗原控制EB病毒感染。当从这些伯基特淋巴瘤细胞系中回收的EB病毒用于转化血清阴性供体的外周血淋巴细胞时，产生的淋巴母细胞系（LCLs）均为LYDMA阳性。这表明伯基特淋巴瘤细胞系的LYDMA阴性特性不是常驻病毒基因组发生突变的结果，而是这些细胞中的一种特定适应性变化，这可能使得肿瘤在发展过程中能够逃避宿主的免疫监视。对六个LYDMA阴性和两个LYDMA阳性的伯基特淋巴瘤细胞系及其相应的淋巴母细胞系中EB病毒基因表达的比较显示，一些伯基特淋巴瘤细胞系并未表达所有经典的与EB病毒潜伏转化相关的病毒基因产物。八个细胞系中有四个显示潜伏膜蛋白（LMP）和/或EB核抗原EBNA 2的表达受限。因此，在一些伯基特淋巴瘤细胞系中似乎存在EB病毒基因调控的新水平。细胞系中EB病毒基因的表达模式与这些细胞系已知的对T细胞杀伤的敏感性没有任何相关性。
