Grumet F C, Fish L, Moossazadeh J, Ness D, Duceman B W
Mol Biol Med. 1983 Dec;1(5):501-9.
A DNA probe specific for the HLA-B locus has been isolated from a broadly cross-reactive HLA class I genomic clone. Locus specificity of the probe appears to be derived primarily from a stretch of approximately 180 nucleotides comprising the last (7th) intron of the original B7 gene. Use of the probe to analyze Southern blots of genomic DNA from unrelated individuals provides the first direct demonstration of intragenic localization of an HLA allele-specific restriction endonuclease site. Availability of this probe should make practicable the study of HLA-B locus restriction fragment length polymorphism as genetic markers of disease susceptibility, and should provide a model for developing probes specific for other HLA class I loci.
一种针对HLA - B基因座的DNA探针已从一个具有广泛交叉反应性的HLA I类基因组克隆中分离出来。该探针的基因座特异性似乎主要源自一段约180个核苷酸的片段，该片段包含原始B7基因的最后一个（第7个）内含子。使用该探针分析无关个体基因组DNA的Southern印迹，首次直接证明了HLA等位基因特异性限制性内切酶位点的基因内定位。这种探针的可用性应使研究HLA - B基因座限制性片段长度多态性作为疾病易感性的遗传标记变得切实可行，并应为开发针对其他HLA I类基因座的探针提供一个模型。
