Campbell S M, Rosen J M, Hennighausen L G, Strech-Jurk U, Sippel A E
Nucleic Acids Res. 1984 Nov 26;12(22):8685-97. doi: 10.1093/nar/12.22.8685.
Whey acidic protein (WAP), a hormonally-regulated 14,000 dalton cysteine-rich protein, is the principal whey protein found in rodent milk. Genomic clones encompassing both the 2.8 Kb rat and 3.3 Kb mouse WAP genes have been characterized. The genes consist of four exons and three introns. The middle two exons encode the two cysteine-rich regions which probably form separate protein domains. Homology in the 5' flanking DNA of the mouse and rat extends at least 325 bp upstream of the putative CAP site, including a precisely conserved stretch of 50 bp around the unusual TATA and CAAT sites. The homology previously observed between the 3' noncoding sequences of the rat and mouse mRNAs extends at least 20 bp into the 3' flanking region. Several potential glucocorticoid receptor binding sites have been found in the 5' flanking region of the WAP gene. The conservation of the 5' flanking region of the WAP genes may be related to regulation of expression of WAP by peptide and/or steroid hormones.
乳清酸性蛋白（WAP）是一种受激素调节的、分子量为14,000道尔顿的富含半胱氨酸的蛋白质，是啮齿动物乳汁中主要的乳清蛋白。包含2.8kb大鼠和3.3kb小鼠WAP基因的基因组克隆已被鉴定。这些基因由四个外显子和三个内含子组成。中间的两个外显子编码两个富含半胱氨酸的区域，这两个区域可能形成独立的蛋白质结构域。小鼠和大鼠5'侧翼DNA的同源性在假定的CAP位点上游至少延伸325bp，包括在不寻常的TATA和CAAT位点周围一段精确保守的50bp序列。先前在大鼠和小鼠mRNA的3'非编码序列之间观察到的同源性在3'侧翼区域至少延伸20bp。在WAP基因的5'侧翼区域发现了几个潜在的糖皮质激素受体结合位点。WAP基因5'侧翼区域的保守性可能与肽和/或类固醇激素对WAP表达的调节有关。
