小鼠α-乳白蛋白的放射免疫测定法。

A radioimmunoassay for mouse alpha-lactalbumin.

作者信息

Zamierowski M M, Ebner K E

出版信息

J Immunol Methods. 1980;36(3-4):211-20. doi: 10.1016/0022-1759(80)90126-x.

Abstract

alpha-Lactalbumin has been purified from mouse milk by the use of ammonium sulfate precipitation, Bio-Gel P-100 chromatography and DEAE-cellulose chromatography. Mouse alpha-lactalbumin exists as two major charge forms with the same molecular weight. Both charge forms have been used in the development of a radioimmunoassay to mouse alpha-lactalbumin. The assay is specific for mouse alpha-lactalbumin, reacting identically with both forms, and sensitive with a minimal detectable concentration of 0.35 ng. alpha-Lactalbumin induced in mouse mammary gland explants has been measured with the use of the radioimmunoassay and is detectable in both the explants and media of the cultures.

摘要

通过硫酸铵沉淀、Bio-Gel P-100柱色谱和DEAE-纤维素柱色谱法从鼠乳中纯化出α-乳白蛋白。小鼠α-乳白蛋白以两种主要电荷形式存在，分子量相同。两种电荷形式均用于开发针对小鼠α-乳白蛋白的放射免疫测定法。该测定法对小鼠α-乳白蛋白具有特异性，对两种形式的反应相同，且灵敏度高，最低可检测浓度为0.35 ng。利用放射免疫测定法测定了小鼠乳腺外植体中诱导产生的α-乳白蛋白，并且在培养物的外植体和培养基中均可检测到。