Harris J D, Blum H, Scott J, Traynor B, Ventura P, Haase A
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7212-5. doi: 10.1073/pnas.81.22.7212.
Under permissive conditions of growth in tissue culture, the retrovirus visna multiples over the course of a few days to high titer and kills the host cell. We show that in this lytic life cycle, viral DNA is tightly associated with, but not covalently linked to, chromosomal DNA. This finding provides explanations for a number of the unusual properties of the lentivirus subfamily of retroviruses, and suggests potential mechanisms for the block in virus gene expression in vivo responsible for the slow infection in nature.
在组织培养的宽松生长条件下，逆转录病毒维斯纳病毒在几天内增殖至高滴度并杀死宿主细胞。我们发现，在这种裂解性生命周期中，病毒DNA与染色体DNA紧密相关，但并非共价连接。这一发现解释了逆转录病毒慢病毒亚科的许多异常特性，并提示了体内病毒基因表达受阻导致自然状态下慢感染的潜在机制。
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