磷酸核糖焦磷酸合成酶的分光光度测定法。
A spectrophotometric assay of phosphoribosyl pyrophosphate synthetase.
作者信息
Braven J, Hardwell T R, Seddon R, Whittaker M
出版信息
Ann Clin Biochem. 1984 Sep;21 ( Pt 5):366-71. doi: 10.1177/000456328402100505.
PMID:6095727
Abstract
A simple spectrophotometric assay of phosphoribosyl pyrophosphate synthetase from human erythrocytes is described. The kinetic properties of the enzyme have been determined and are in good agreement with published results. Conditions for storing haemolysates at -20 degrees C are described. The method is suitable for semi-automation.
摘要
本文描述了一种简单的分光光度法，用于检测人红细胞中的磷酸核糖焦磷酸合成酶。已测定该酶的动力学性质，其结果与已发表的结果高度吻合。文中还介绍了在-20℃下储存溶血产物的条件。该方法适用于半自动化操作。
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