一种用于测定人血清中血管紧张素转换酶的动力学荧光测定法。

A kinetic fluorimetric assay for the measurement of angiotensin-converting enzyme in human serum.

作者信息

Maguire G A, Price C P

出版信息

Ann Clin Biochem. 1984 Sep;21 ( Pt 5):372-7. doi: 10.1177/000456328402100506.

Abstract

The development and assay performance of a direct kinetic fluorimetric assay for angiotensin-converting enzyme is described. It is based on the substrate developed by Carmel et al. (Clin Chim Acta 1979; 93: 215-20), and applied to a microcentrifugal analyser with fluorescence optics. The method is sufficiently sensitive and precise to detect both normal and pathological activities; the method is linear up to at least three times the upper limit of the reference range. The method correlates well with an established method. Because of interference, the method is unsuitable for use with icteric or haemolysed samples.

摘要

本文描述了一种用于血管紧张素转换酶的直接动力学荧光测定法的开发及其分析性能。该方法基于Carmel等人开发的底物（《临床化学学报》，1979年；93：215 - 20），并应用于配备荧光光学器件的微量离心分析仪。该方法灵敏度和精密度足以检测正常和病理活性；该方法在至少参考范围上限的三倍以内呈线性。该方法与已建立的方法相关性良好。由于存在干扰，该方法不适用于黄疸或溶血样本。