Dunn J K, Searcy G P, Hirsch V M
Can J Comp Med. 1984 Oct;48(4):349-53.
The preparation of a feline Coombs serum (rabbit antifeline gamma globulin) is described. The direct antiglobulin test using this serum was performed on 20 anemic and 20 healthy control cats. Red cell membrane antibodies were detected in cats with feline leukemia virus infection and in others with inflammatory and neoplastic diseases. A low titre of cold agglutinating antibody was present in a high proportion of the control cats. Positive direct antiglobulin tests were noted in cats without overt hemolytic disease. It was concluded that the direct antiglobulin test in anemic cats has certain diagnostic limitations. A positive reaction should be interpreted cautiously especially when there is no clinical or laboratory evidence to support a diagnosis of autoimmune hemolytic anemia.
本文描述了猫库姆斯血清（兔抗猫γ球蛋白）的制备方法。使用该血清对20只贫血猫和20只健康对照猫进行了直接抗球蛋白试验。在感染猫白血病病毒的猫以及其他患有炎症和肿瘤性疾病的猫中检测到红细胞膜抗体。高比例的对照猫存在低滴度的冷凝集抗体。在无明显溶血性疾病的猫中也观察到直接抗球蛋白试验阳性。得出的结论是，贫血猫的直接抗球蛋白试验有一定的诊断局限性。尤其是在没有临床或实验室证据支持自身免疫性溶血性贫血诊断的情况下，对阳性反应应谨慎解释。
