Craig N L, Nash H A
Cell. 1984 Dec;39(3 Pt 2):707-16. doi: 10.1016/0092-8674(84)90478-1.
E. coli integration host factor (IHF) both participates directly in phage lambda site-specific recombination and regulates the expression of phage and bacterial genes. Using protection from nuclease and chemical attack as an assay, we examined the interaction of IHF with DNA. We found that IHF is a specific DNA binding protein that interacts with three distinct segments of attP, the recombination site carried by phage lambda. We also found that specific IHF binding sites are located in non-att DNA. Several non-att IHF binding sites that we have identified are adjacent to genes whose expression is altered in IHF mutants. From comparison of the sequences protected by IHF, we suggest that the critical determinant in specific IHF-DNA interaction is contained in the sequence T.PyAA...PuTTGaT.A.PuTT...PyAACtA.
大肠杆菌整合宿主因子（IHF）既直接参与噬菌体λ位点特异性重组，又调控噬菌体和细菌基因的表达。我们以核酸酶保护和化学攻击实验为分析方法，研究了IHF与DNA的相互作用。我们发现，IHF是一种特异性DNA结合蛋白，它与噬菌体λ携带的重组位点attP的三个不同片段相互作用。我们还发现，特异性IHF结合位点位于非att DNA中。我们鉴定出的几个非att IHF结合位点与在IHF突变体中表达发生改变的基因相邻。通过比较受IHF保护的序列，我们认为特异性IHF-DNA相互作用的关键决定因素包含在序列T.PyAA...PuTTGaT.A.PuTT...PyAACtA中。
