Manara G C, De Panfilis G, Ferrari C, Scandroglio R
J Immunol Methods. 1984 Dec 14;75(1):189-92. doi: 10.1016/0022-1759(84)90238-2.
A method for better characterization of mononuclear cell subpopulations using detection of 2 surface antigens simultaneously in electron microscopy was developed with immunoperoxidase-immunogold double labelling. Monoclonal antibodies of IgG and IgM classes were used in the first step, and colloidal gold-labelled anti-mouse IgG antibody and peroxidase-labelled anti-mouse IgM antibody (mu chain-specific) in the second step. Immunoelectronmicroscopy with such double labelling improves ultrastructural analysis of mononuclear cell subpopulations.
通过免疫过氧化物酶-免疫金双重标记，开发了一种在电子显微镜下同时检测两种表面抗原以更好地表征单核细胞亚群的方法。第一步使用IgG和IgM类别的单克隆抗体，第二步使用胶体金标记的抗小鼠IgG抗体和过氧化物酶标记的抗小鼠IgM抗体（μ链特异性）。这种双重标记的免疫电子显微镜改善了单核细胞亚群的超微结构分析。
