Mouton C, Lamonde L
Can J Microbiol. 1984 Aug;30(8):1008-13. doi: 10.1139/m84-157.
Colloidal gold particles 3-6 nm in diameter were prepared and stabilized with the IgG fraction of polyspecific rabbit antisera produced against four different oral bacteria. The immunogold markers were used in homologous reactions to label the bacteria in a preembedding procedure for electron microscopy. An indirect immunofluorescence procedure was concurrently used to optimize the labelling conditions before observation with the electron microscope. The immunogold markers labelled fibrillar structures extending outward 50-275 nm from the Gram-positive cell envelopes and a fuzzy 5-10 nm thick capsulelike layer on the outer aspect of Bacteroides gingivalis. The immunogold method appears to be a simple, rapid, and inexpensive procedure suitable for the study of bacterial surface antigens and can be upgraded with the use of monospecific antibodies.
制备了直径为3 - 6纳米的胶体金颗粒，并用针对四种不同口腔细菌产生的多特异性兔抗血清的IgG组分进行稳定化处理。免疫金标记物用于同源反应，在电子显微镜的包埋前程序中标记细菌。在用电镜观察之前，同时使用间接免疫荧光程序优化标记条件。免疫金标记物标记了从革兰氏阳性菌细胞壁向外延伸50 - 275纳米的纤维状结构，以及牙龈拟杆菌外侧模糊的5 - 10纳米厚的类荚膜层。免疫金方法似乎是一种简单、快速且廉价的程序，适用于研究细菌表面抗原，并且可以通过使用单特异性抗体进行改进。
