Asano Y, Albrecht P, Behr D E, Neff B J, Vickers J H, Rastogi S C
J Med Virol. 1984;14(4):305-12. doi: 10.1002/jmv.1890140403.
Thirty susceptible rhesus monkeys were inoculated with cell-free varicella-zoster virus strain OKA or strain KMcC. Both wild and attenuated strains were used. No clinical signs characteristic of human varicella were seen in any of the animals. Virus was not isolated from throat swabs, blood, or cerebrospinal fluid. Antibodies were measured by an enhanced plaque neutralization test. The wild and attenuated OKA strains produced comparable levels of antibodies for 3 months after inoculation. Attenuated KMcC strains produced lower titers than the wild strain. On rechallenge 3 months after primary inoculation animals boostered with the attenuated OKA strain developed significantly higher antibody titers than animals receiving the wild strain. Animals primed and challenged with the attenuated KMcC strains showed significantly lower antibody titers than animals which received the wild strain. The results indicate that the immunogenicity of attenuated OKA and KMcC strains in rhesus monkeys parallels the experience obtained with these strains in humans.
30只易感恒河猴接种了无细胞水痘 - 带状疱疹病毒OKA株或KMcC株。使用了野生株和减毒株。在任何动物中均未观察到人类水痘的特征性临床症状。未从咽拭子、血液或脑脊液中分离出病毒。通过增强空斑中和试验检测抗体。接种后3个月，野生型和减毒OKA株产生的抗体水平相当。减毒KMcC株产生的滴度低于野生株。在初次接种3个月后再次攻击时，用减毒OKA株加强免疫的动物产生的抗体滴度明显高于接受野生株的动物。用减毒KMcC株进行初次免疫和攻击的动物显示出的抗体滴度明显低于接受野生株的动物。结果表明，减毒OKA株和KMcC株在恒河猴中的免疫原性与在人类中使用这些毒株的经验相似。
