Cosman D, Cerretti D P, Larsen A, Park L, March C, Dower S, Gillis S, Urdal D
Nature. 1984;312(5996):768-71. doi: 10.1038/312768a0.
T lymphocytes, essential for the generation of a normal immune response, require the presence of the lymphokine interleukin-2 (IL-2) in order to proliferate. Cells that respond to IL-2 possess a surface receptor glycoprotein specific for this lymphokine. We have recently purified and chemically characterized the IL-2 receptor from both phytohaemagglutinin-activated human T cells and the human T-cell lymphoma HUT-102 (ref. 5). From the NH2-terminal protein sequence obtained in that study, we have now used synthetic oligonucleotides to probe a complementary DNA library, prepared from HUT-102 messenger RNA, for the presence of cDNA clones that might code for the IL-2 receptor. Two cDNA clones were isolated which had closely related DNA sequences. Interestingly, only one coded for an active receptor when transfected into COS-7 cells. This clone contained a 216-base pair (bp) insert that was not present in the other clone. The insert was flanked by an 8-bp direct repeat reminiscent of a transposable element, and appeared to code for a region of marked structural homology to the NH2-terminal region of the receptor molecule.
T淋巴细胞是产生正常免疫反应所必需的，它需要淋巴因子白细胞介素-2（IL-2）的存在才能增殖。对IL-2有反应的细胞具有针对这种淋巴因子的表面受体糖蛋白。我们最近从植物血凝素激活的人T细胞和人T细胞淋巴瘤HUT-102中纯化并对IL-2受体进行了化学表征（参考文献5）。根据该研究中获得的NH2末端蛋白质序列，我们现在使用合成寡核苷酸探测从HUT-102信使RNA制备的互补DNA文库，以寻找可能编码IL-2受体的cDNA克隆。分离出两个具有密切相关DNA序列的cDNA克隆。有趣的是，当转染到COS-7细胞中时，只有一个编码活性受体。该克隆包含一个216碱基对（bp）的插入片段，另一个克隆中不存在该片段。该插入片段两侧是一个8bp的直接重复序列，让人联想到转座元件，并且似乎编码与受体分子NH2末端区域具有明显结构同源性的区域。
