Koob G F, Swerdlow N, Seeligson M, Eaves M, Sutton R, Rivier J, Vale W
Neuroendocrinology. 1984 Nov;39(5):459-64. doi: 10.1159/000124021.
Corticotropin-releasing factor injected intracerebroventricularly in a dose of 1 microgram produced a prolonged locomotor activation (3 h) in rats previously habituated to the test cage environment. This activation was reversed by alpha-flupenthixol (intraperitoneally), a dopamine receptor antagonist, only at cataleptic doses and not at all by naloxone (subcutaneously) in doses of 0.02-5.0 mg/kg. The effective dose 50% (ED50) for the alpha-flupenthixol reversal of locomotor activity induced by corticotropin-releasing factor was 0.13 mg/kg; similar to the 0.14 mg/kg ED50 needed to reverse the locomotor activation produced by caffeine (10 mg/kg s.c.). The ED50 necessary to reverse amphetamine (0.75 mg/kg s.c.) locomotion with this drug was 0.07 mg/kg. The results suggest that the corticotropin-releasing factor acts independently of direct activation of the dopamine or opioid peptide systems.
向预先适应测试笼环境的大鼠脑室内注射1微克剂量的促肾上腺皮质激素释放因子，会产生持续较长时间的运动激活（3小时）。这种激活仅在致僵剂量下可被多巴胺受体拮抗剂α-氟哌噻吨（腹腔注射）逆转，而皮下注射剂量为0.02 - 5.0毫克/千克的纳洛酮则完全不能逆转。α-氟哌噻吨逆转促肾上腺皮质激素释放因子诱导的运动活动的半数有效剂量（ED50）为0.13毫克/千克；这与逆转咖啡因（皮下注射10毫克/千克）产生的运动激活所需的0.14毫克/千克ED50相似。用该药物逆转苯丙胺（皮下注射0.75毫克/千克）运动的必要ED50为0.07毫克/千克。结果表明，促肾上腺皮质激素释放因子的作用独立于多巴胺或阿片肽系统的直接激活。
