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蓝藻鱼腥藻藻蓝蛋白操纵子的克隆及表达的光调节

Cloning and light regulation of expression of the phycocyanin operon of the cyanobacterium Anabaena.

作者信息

Belknap W R, Haselkorn R

出版信息

EMBO J. 1987 Apr;6(4):871-84. doi: 10.1002/j.1460-2075.1987.tb04833.x.

DOI:10.1002/j.1460-2075.1987.tb04833.x
PMID:3109890
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553477/
Abstract

The biliprotein phycocyanin (PC) is a major constituent of the light-harvesting apparatus of cyanobacteria and red algae. A DNA fragment encoding the beta and alpha subunits of PC was isolated from a genomic library of the cyanobacterium Anabaena 7120 DNA. The single-copy PC genes are part of a larger operon which consists of five open reading frames (ORFs) encoding, in order, the beta and alpha subunits of PC, two linker polypeptides associated with PC in phycobilisome rods, and a fifth ORF, which may encode a linker polypeptide involved in attachment of the phycobilisome rod to the core of the structure. The operon yields three major transcripts, the first of which (1.4 kb) encodes only the PC subunits. A second (3.6 kb) encodes all five ORFs, and appears to arise from partial read-through of a terminator following the PC subunit genes. The third transcript (1.4 kb) encodes the last two ORFs. The relative levels of the three transcripts in vivo are modulated by light intensity, but they are not altered by the removal of fixed nitrogen from the growth medium. The site of light regulation appears to be the terminator following the PC genes, rather than a promoter.

摘要

藻胆蛋白藻蓝蛋白（PC）是蓝细菌和红藻光捕获装置的主要成分。从蓝细菌鱼腥藻7120 DNA的基因组文库中分离出编码PC的β和α亚基的DNA片段。单拷贝的PC基因是一个更大操纵子的一部分，该操纵子由五个开放阅读框（ORF）组成，依次编码PC的β和α亚基、与藻胆体棒中PC相关的两个连接多肽，以及第五个ORF，它可能编码参与藻胆体棒与结构核心连接的连接多肽。该操纵子产生三种主要转录本，第一种（1.4 kb）仅编码PC亚基。第二种（3.6 kb）编码所有五个ORF，似乎是由于PC亚基基因后面的终止子部分通读产生的。第三种转录本（1.4 kb）编码最后两个ORF。这三种转录本在体内的相对水平受光强度调节，但从生长培养基中去除固定氮不会改变它们的水平。光调节的位点似乎是PC基因后面的终止子，而不是启动子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/0be96fba412e/emboj00244-0053-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/1fd593fb79b3/emboj00244-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/b1e5cf6d02e9/emboj00244-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/f75a8840ad24/emboj00244-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/8b896355d9fc/emboj00244-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/fab11ef3a796/emboj00244-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/847a210ecd64/emboj00244-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/0be96fba412e/emboj00244-0053-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/1fd593fb79b3/emboj00244-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/b1e5cf6d02e9/emboj00244-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/f75a8840ad24/emboj00244-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/8b896355d9fc/emboj00244-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/fab11ef3a796/emboj00244-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/847a210ecd64/emboj00244-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c965/553477/0be96fba412e/emboj00244-0053-b.jpg

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