LaPolla R J, Mayne K M, Davidson N
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7970-4. doi: 10.1073/pnas.81.24.7970.
A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the delta subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector lambda 10 from membrane-associated poly(A)+ RNA from BC3H-1 mouse cells. Surprisingly, the delta clone was selected by hybridization with cDNA encoding the gamma subunit of the Torpedo AcChoR. The nucleotide sequence of the mouse cDNA clone contains an open reading frame of 520 amino acids. This amino acid sequence exhibits 59% and 50% sequence homology to the Torpedo AcChoR delta and gamma subunits, respectively. However, the mouse nucleotide sequence has several stretches of high homology with the Torpedo gamma subunit cDNA, but not with delta. The mouse protein has the same general structural features as do the Torpedo subunits. It is encoded by a 3.3-kilobase mRNA. There is probably only one, but at most two, chromosomal genes coding for this or closely related sequences.
已分离出一个小鼠cDNA克隆，其包含一种与电鳐乙酰胆碱受体（AcChoR）δ亚基高度同源的蛋白质的完整编码区。该cDNA文库是用来自BC3H - 1小鼠细胞膜相关的聚腺苷酸加尾（poly(A)+）RNA构建于λ10载体中。令人惊讶的是，该δ克隆是通过与编码电鳐AcChoRγ亚基的cDNA杂交筛选出来的。小鼠cDNA克隆的核苷酸序列包含一个520个氨基酸的开放阅读框。该氨基酸序列与电鳐AcChoRδ亚基和γ亚基的序列同源性分别为59%和50%。然而，小鼠核苷酸序列与电鳐γ亚基cDNA有几段高度同源，但与δ亚基cDNA没有。小鼠蛋白质具有与电鳐亚基相同的一般结构特征。它由一个3.3千碱基的mRNA编码。可能只有一个，但最多两个染色体基因编码该序列或密切相关的序列。
