Petrenko A Iu, Belous A M, Marshanskiĭ V N, Novgorodov S A, Iaguzhinskiĭ L S
Ukr Biokhim Zh (1978). 1984 Nov-Dec;56(6):656-60.
The reasons of ion transport induction observed after freezing-thawing of the rat liver mitochondria were investigated. It is found that a fall in the membrane potential value, an increase in the respiration rate and K+ ion release from mitochondria to the incubation medium with phosphate are averted when succinate is replaced by the Kreb's cycle substrates and when ionol (an antioxidant) or nupercain (an inhibitor of mitochondrial phospholipase A2) are incorporated into the incubation medium. The induction of ion transport is caused by the activation of peroxide processes and of lipolysis associated with them. It is supposed that under experimental conditions after freezing-thawing a degree of pyridine nucleotides and glutathione reduction lowers, that in its turn leads to the inhibition of the glutathione-peroxidase activity and development of peroxide processes.
研究了大鼠肝脏线粒体冻融后离子转运诱导的原因。结果发现，当用三羧酸循环底物替代琥珀酸，以及将离子醇（一种抗氧化剂）或奴夫卡因（一种线粒体磷脂酶A2抑制剂）加入孵育介质时，膜电位值下降、呼吸速率增加以及钾离子从线粒体释放到含磷酸盐的孵育介质中的情况均可避免。离子转运的诱导是由过氧化物过程及其相关的脂解作用的激活引起的。据推测，在冻融后的实验条件下，吡啶核苷酸和谷胱甘肽的还原程度降低，这进而导致谷胱甘肽过氧化物酶活性受到抑制以及过氧化物过程的发展。
