平面脂质双分子层中单个氨氯地平敏感型Na⁺通道的饱和行为。
Saturation behavior of single, amiloride-sensitive Na+ channels in planar lipid bilayers.
作者信息
Olans L, Sariban-Sohraby S, Benos D J
出版信息
Biophys J. 1984 Dec;46(6):831-5. doi: 10.1016/S0006-3495(84)84082-5.
Abstract
Single epithelial Na+ channels incorporated into planar lipid bilayers were studied to determine the effects of Na concentration on its own conductance. Amiloride-sensitive Na+ channels were obtained from apical membrane vesicles made from A6 cells, a continuous epithelial cell-line derived from amphibian kidney. Single-channel conductance was found to be a saturable function of Na+ concentration, with a Michaelis constant of approximately 17 or 47 mM, for a Gmax of approximately 4 or 44 pS, respectively.
摘要
研究了整合到平面脂质双分子层中的单个上皮钠通道，以确定钠浓度对其自身电导的影响。氨氯地平敏感的钠通道取自A6细胞制成的顶端膜囊泡，A6细胞是一种源自两栖动物肾脏的连续上皮细胞系。发现单通道电导是钠浓度的饱和函数，米氏常数分别约为17或47 mM，最大电导分别约为4或44 pS。
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