Balàzs C, Farid N R
Clin Invest Med. 1984;7(3):157-60.
Evidence concerning the role of hyperthyroidism per se in suppressor cell dysfunction in Graves' disease is conflicting. To investigate this issue, we studied the effect of triiodothyronine (T3) administration to ten healthy volunteers for 7 days on spontaneous (short-lived) and Concanavalin A (Con A)-induced suppressor cell activities. The short-lived suppressor cell index is a ratio of [3H] thymidine incorporation induced by Concanavalin A in lymphocytes preincubated for 24 h (to remove adherent cells) to that of non-incubated lymphocytes; the more active the adherent suppressor cells, the higher this index. The mean short-lived suppressor cell index rose from a mean of 2.46 +/- 0.15 (+/- S.D.) before T3 treatment to 3.29 +/- 0.46 (p less than 0.01) after 7 days of T3. Concanavalin A-induced suppressor cell activity, which measures the potential suppressive activity of lymphocytes triggered by Con A is expressed as the percentage decrease in [3H] thymidine incorporation by lymphocytes cultured with Con A for 72 h in relation to that by fresh lymphocytes. Con A-induced suppressor cell activity also improved from 55.8% +/- 2.5 before T3 treatment to 62.4% +/- 1.9 (p less than 0.05) at 7 days of this treatment. These studies show that within the time limitations of the study, T3 appears to enhance short-lived and Concanavalin A-induced suppressor cell activities.
关于甲状腺功能亢进本身在格雷夫斯病抑制细胞功能障碍中所起作用的证据相互矛盾。为了研究这个问题，我们对10名健康志愿者进行了为期7天的三碘甲状腺原氨酸（T3）给药，并观察其对自发（短期）和刀豆蛋白A（Con A）诱导的抑制细胞活性的影响。短期抑制细胞指数是指在预先孵育24小时（以去除贴壁细胞）的淋巴细胞中，由刀豆蛋白A诱导的[3H]胸腺嘧啶核苷掺入量与未孵育淋巴细胞的该掺入量之比；贴壁抑制细胞活性越高，该指数越高。T3治疗前短期抑制细胞指数的平均值为2.46±0.15（±标准差），T3治疗7天后升至3.29±0.46（p<0.01）。刀豆蛋白A诱导的抑制细胞活性，用于衡量由Con A触发的淋巴细胞的潜在抑制活性，以与新鲜淋巴细胞相比，在含有Con A的情况下培养72小时的淋巴细胞中[3H]胸腺嘧啶核苷掺入量的减少百分比来表示。刀豆蛋白A诱导的抑制细胞活性也从T3治疗前的55.8%±2.5提高到该治疗7天时的62.4%±1.9（p<0.05）。这些研究表明，在本研究的时间限制内，T3似乎增强了短期和刀豆蛋白A诱导的抑制细胞活性。
