Marians K J
CRC Crit Rev Biochem. 1984;17(2):153-215. doi: 10.3109/10409238409113604.
This review stresses recent developments in the in vitro study of DNA replication in prokaryotes. New insights into the enzymological mechanisms of initiation and elongation of leading and lagging strand DNA synthesis in ongoing studies are emphasized. Data from newly developed systems, such as those replicating oriC containing DNA or which are dependent on the lambda, O, and P proteins, are presented and the information compared to existing mechanisms. Evidence bearing on the coupling of DNA synthesis on both parental strands through protein-protein interactions and on the turnover of the elongation systems are analyzed. The structure of replication origins, and how their tertiary structure affects recognition and interaction with the various replication proteins is discussed.
本综述着重介绍了原核生物DNA复制体外研究的最新进展。强调了在正在进行的研究中对前导链和滞后链DNA合成起始及延伸的酶学机制的新见解。展示了来自新开发系统的数据，例如那些复制含oriC DNA的系统或依赖λ、O和P蛋白的系统，并将这些信息与现有机制进行了比较。分析了通过蛋白质-蛋白质相互作用在两条亲代链上进行DNA合成的偶联以及延伸系统周转的相关证据。讨论了复制起点的结构，以及它们的三级结构如何影响与各种复制蛋白的识别和相互作用。
