细菌生长需要在重组中间体处组装复制叉。
Replication fork assembly at recombination intermediates is required for bacterial growth.
作者信息
Liu J, Xu L, Sandler S J, Marians K J
机构信息
Molecular Biology Graduate Program, Cornell University Graduate School of Medical Sciences, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3552-5. doi: 10.1073/pnas.96.7.3552.
Abstract
PriA, a 3' --> 5' DNA helicase, directs assembly of a primosome on some bacteriophage and plasmid DNAs. Primosomes are multienzyme replication machines that contribute both the DNA-unwinding and Okazaki fragment-priming functions at the replication fork. The role of PriA in chromosomal replication is unclear. The phenotypes of priA null mutations suggest that the protein participates in replication restart at recombination intermediates. We show here that PriA promotes replication fork assembly at a D loop, an intermediate formed during initiation of homologous recombination. We also show that DnaC810, encoded by a naturally arising intergenic suppressor allele of the priA2::kan mutation, bypasses the need for PriA during replication fork assembly at D loops in vitro. These findings underscore the essentiality of replication fork restart at recombination intermediates under normal growth conditions in bacteria.
摘要
PriA是一种3'→5' DNA解旋酶，可指导在某些噬菌体和质粒DNA上组装引发体。引发体是多酶复制机器，在复制叉处发挥DNA解旋和冈崎片段引发功能。PriA在染色体复制中的作用尚不清楚。priA缺失突变的表型表明该蛋白参与重组中间体处的复制重启。我们在此表明，PriA促进D环处的复制叉组装，D环是同源重组起始过程中形成的中间体。我们还表明，priA2::kan突变的天然发生的基因间抑制等位基因编码的DnaC810在体外D环处的复制叉组装过程中绕过了对PriA的需求。这些发现强调了在细菌正常生长条件下重组中间体处复制叉重启的必要性。
相似文献
1
Replication fork assembly at recombination intermediates is required for bacterial growth.细菌生长需要在重组中间体处组装复制叉。
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3552-5. doi: 10.1073/pnas.96.7.3552.
2
Purification and characterization of DnaC810, a primosomal protein capable of bypassing PriA function.能够绕过PriA功能的引发体蛋白DnaC810的纯化与特性分析
J Biol Chem. 2000 Mar 17;275(11):8196-205. doi: 10.1074/jbc.275.11.8196.
3
Differential suppression of priA2::kan phenotypes in Escherichia coli K-12 by mutations in priA, lexA, and dnaC.在大肠杆菌K-12中，priA、lexA和dnaC基因的突变对priA2::kan表型的差异抑制作用
Genetics. 1996 May;143(1):5-13. doi: 10.1093/genetics/143.1.5.
4
PriA: at the crossroads of DNA replication and recombination.PriA：处于DNA复制与重组的交叉点。
Prog Nucleic Acid Res Mol Biol. 1999;63:39-67. doi: 10.1016/s0079-6603(08)60719-9.
5
Duplex opening by primosome protein PriA for replisome assembly on a recombination intermediate.引发体蛋白PriA打开双链，以便在重组中间体上组装复制体。
J Mol Biol. 1999 Jun 11;289(3):503-16. doi: 10.1006/jmbi.1999.2783.
6
Escherichia coli and Bacillus subtilis PriA proteins essential for recombination-dependent DNA replication: involvement of ATPase/helicase activity of PriA for inducible stable DNA replication.大肠杆菌和枯草芽孢杆菌中参与依赖重组的DNA复制所必需的PriA蛋白：PriA的ATP酶/解旋酶活性对诱导性稳定DNA复制的作用
Biochimie. 1999 Aug-Sep;81(8-9):847-57. doi: 10.1016/s0300-9084(99)00211-4.
7
Helicase-deficient cysteine to glycine substitution mutants of Escherichia coli replication protein PriA retain single-stranded DNA-dependent ATPase activity. Zn2+ stimulation of mutant PriA helicase and primosome assembly activities.大肠杆菌复制蛋白PriA的解旋酶缺陷型半胱氨酸到甘氨酸取代突变体保留单链DNA依赖性ATP酶活性。Zn2+对突变型PriA解旋酶和引发体组装活性的刺激作用。
J Biol Chem. 1993 Feb 25;268(6):4337-46.
8
Escherichia coli PriA helicase: fork binding orients the helicase to unwind the lagging strand side of arrested replication forks.大肠杆菌PriA解旋酶：叉形结构结合使解旋酶定向，以解开停滞复制叉的滞后链一侧。
J Mol Biol. 2001 Oct 5;312(5):935-47. doi: 10.1006/jmbi.2001.4930.
9
The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes.φX174型引发体的有序组装。I. 中间蛋白质-DNA复合物的分离与鉴定。
J Biol Chem. 1996 Jun 28;271(26):15642-8. doi: 10.1074/jbc.271.26.15642.
10
The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair.DNA复制引发蛋白PriA是同源重组和双链断裂修复所必需的。
J Bacteriol. 1996 Mar;178(5):1258-64. doi: 10.1128/jb.178.5.1258-1264.1996.
引用本文的文献
1
The Bacillus subtilis PriA Winged Helix Domain Is Critical for Surviving DNA Damage.枯草芽孢杆菌 PriA 卷曲螺旋结构域对于耐受 DNA 损伤至关重要。
J Bacteriol. 2022 Mar 15;204(3):e0053921. doi: 10.1128/JB.00539-21. Epub 2022 Jan 10.
2
Single-molecule studies of helicases and translocases in prokaryotic genome-maintenance pathways.原核生物基因组维持途径中解旋酶和移位酶的单分子研究。
DNA Repair (Amst). 2021 Dec;108:103229. doi: 10.1016/j.dnarep.2021.103229. Epub 2021 Sep 20.
3
Single-molecule insight into stalled replication fork rescue in Escherichia coli.单分子视角下的大肠杆菌复制叉停滞挽救。
Nucleic Acids Res. 2021 May 7;49(8):4220-4238. doi: 10.1093/nar/gkab142.
4
Replication Fork Breakage and Restart in Escherichia coli.大肠杆菌中复制叉的断裂与重连。
Microbiol Mol Biol Rev. 2018 Jun 13;82(3). doi: 10.1128/MMBR.00013-18. Print 2018 Sep.
5
Non-Canonical Replication Initiation: You're Fired!非经典复制起始：你被解雇了！
Genes (Basel). 2017 Jan 27;8(2):54. doi: 10.3390/genes8020054.
6
Overexpression of the Replicative Helicase in Escherichia coli Inhibits Replication Initiation and Replication Fork Reloading.大肠杆菌中复制解旋酶的过表达抑制复制起始和复制叉重新加载。
J Mol Biol. 2016 Mar 27;428(6):1068-1079. doi: 10.1016/j.jmb.2016.01.018. Epub 2016 Jan 23.
7
Replication Restart after Replication-Transcription Conflicts Requires RecA in Bacillus subtilis.枯草芽孢杆菌中复制-转录冲突后的复制重启需要RecA。
J Bacteriol. 2015 Jul;197(14):2374-82. doi: 10.1128/JB.00237-15. Epub 2015 May 4.
8
Recombination and replication.重组与复制。
Cold Spring Harb Perspect Biol. 2014 Oct 23;6(11):a016550. doi: 10.1101/cshperspect.a016550.
9
Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.引发体蛋白PriA、PriB和DnaT与DNA结合模式的结构解析
Biomed Res Int. 2014;2014:195162. doi: 10.1155/2014/195162. Epub 2014 Jul 21.
10
DNA damage responses in prokaryotes: regulating gene expression, modulating growth patterns, and manipulating replication forks.原核生物中的DNA损伤反应：调控基因表达、调节生长模式以及操控复制叉。
Cold Spring Harb Perspect Biol. 2013 Nov 1;5(11):a012674. doi: 10.1101/cshperspect.a012674.
本文引用的文献
1
Role of the core DNA polymerase III subunits at the replication fork. Alpha is the only subunit required for processive replication.核心DNA聚合酶III亚基在复制叉处的作用。α是进行性复制所需的唯一亚基。
J Biol Chem. 1998 Jan 23;273(4):2452-7. doi: 10.1074/jbc.273.4.2452.
2
The DNA replication protein PriA and the recombination protein RecG bind D-loops.DNA复制蛋白PriA和重组蛋白RecG可结合D环。
J Mol Biol. 1997 Jul 11;270(2):212-21. doi: 10.1006/jmbi.1997.1120.
3
Differential suppression of priA2::kan phenotypes in Escherichia coli K-12 by mutations in priA, lexA, and dnaC.在大肠杆菌K-12中，priA、lexA和dnaC基因的突变对priA2::kan表型的差异抑制作用
Genetics. 1996 May;143(1):5-13. doi: 10.1093/genetics/143.1.5.
4
The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes.φX174型引发体的有序组装。I. 中间蛋白质-DNA复合物的分离与鉴定。
J Biol Chem. 1996 Jun 28;271(26):15642-8. doi: 10.1074/jbc.271.26.15642.
5
Recombination by replication.通过复制进行重组。
Cell. 1996 May 31;85(5):625-7. doi: 10.1016/s0092-8674(00)81229-5.
6
The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair.DNA复制引发蛋白PriA是同源重组和双链断裂修复所必需的。
J Bacteriol. 1996 Mar;178(5):1258-64. doi: 10.1128/jb.178.5.1258-1264.1996.
7
Coupling of a replicative polymerase and helicase: a tau-DnaB interaction mediates rapid replication fork movement.复制性聚合酶与解旋酶的偶联：tau-DnaB相互作用介导快速复制叉移动。
Cell. 1996 Feb 23;84(4):643-50. doi: 10.1016/s0092-8674(00)81039-9.
8
Phi X174-type primosomal proteins: purification and assay.
Methods Enzymol. 1995;262:507-21. doi: 10.1016/0076-6879(95)62042-7.
9
Escherichia coli PriA protein is essential for inducible and constitutive stable DNA replication.大肠杆菌PriA蛋白对于诱导型和组成型稳定DNA复制至关重要。
EMBO J. 1994 Nov 15;13(22):5338-45. doi: 10.1002/j.1460-2075.1994.tb06868.x.
10
Enzymology of DNA in replication in prokaryotes.原核生物复制过程中DNA的酶学
CRC Crit Rev Biochem. 1984;17(2):153-215. doi: 10.3109/10409238409113604.
Zotero 插件