用吖啶橙致敏并用氩激光照射对膀胱癌细胞（MGH-U1）进行光动力灭活。

Photodynamic inactivation of bladder cancer cells (MGH-U1) sensitized with acridine orange and irradiated by argon laser.

作者信息

Ishikawa S, Nemoto R, Kanoh S, Kobayashi K, Ishizaka S

出版信息

Tohoku J Exp Med. 1984 Nov;144(3):265-71. doi: 10.1620/tjem.144.265.

DOI:10.1620/tjem.144.265

Abstract

Photodynamic inactivation of bladder cancer cells (MGH-U1) was investigated in order to apply laser therapy to the treatment of bladder cancer. After stained with acridine orange (AO), the material cells were irradiated with argon laser. After 24 hr incubation the survival was counted with a hemocytometer. The number of stained cells showed a less than 5% decrease and the number of irradiated (for 15 min) unstained cells showed no decrease compared with untreated cells. Participation of singlet oxygen process in inactivation of MGH-U1 cells was confirmed by the use of D2O and NaN3. The result shows that argon laser at the low intensity and with short irradiation time has a sufficient cytocydal effect, suggesting the usefulness of photodynamic inactivation of argon laser with topical use of acridine orange in the treatment of bladder cancer.

摘要

为了将激光疗法应用于膀胱癌治疗，对膀胱癌细胞（MGH-U1）的光动力失活进行了研究。在用吖啶橙（AO）染色后，用氩激光照射细胞。孵育24小时后，用血细胞计数器计数存活细胞。与未处理的细胞相比，染色细胞数量减少不到5%，照射（15分钟）的未染色细胞数量没有减少。通过使用重水（D2O）和叠氮化钠（NaN3）证实了单线态氧过程参与MGH-U1细胞的失活。结果表明，低强度、短照射时间的氩激光具有足够的细胞杀伤作用，提示在膀胱癌治疗中局部使用吖啶橙的氩激光光动力失活具有实用性。