Tatsuta M, Yamamura H, Yamamoto R, Ichii M, Noguchi S, Iishi H, Mishima H, Hattori T, Okuda S
Eur J Cancer Clin Oncol. 1984 Apr;20(4):543-52. doi: 10.1016/0277-5379(84)90241-4.
The phototoxicity of acridine orange and argon laser irradiation on Walker carcinosarcoma 256 stomach tumors was studied. Wistar strain rats bearing stomach tumors 4-6 mm in diameter 5-10 days after their implantation were injected intraperitoneally with 40 mg/kg of acridine orange 2 hr before irradiation. Then the forestomach was opened and the tumors were exposed to the argon laser at 488 nm at an intensity of 15 mW/cm2 for 20 min. Tumors in rats treated with acridine orange were brightly fluorescent during irradiation. No marked temperature rise was detected during irradiation. Argon irradiation significantly prolonged the survival of rats treated with acridine orange. Histologically, complete or partial tumor necrosis was observed, with sparing of surrounding mucosa, in rats treated by the combination therapy. Phase-contrast and electron microscopy showed that cytotoxicity was mediated by changes in the cell, nuclear and lysosomal membranes. Neither the dye nor laser alone had any effect.
研究了吖啶橙与氩激光照射对Walker癌肉瘤256胃肿瘤的光毒性。在植入直径4 - 6毫米胃肿瘤5 - 10天后的Wistar品系大鼠，在照射前2小时腹腔注射40毫克/千克吖啶橙。然后打开前胃，将肿瘤暴露于波长488纳米、强度为15毫瓦/平方厘米的氩激光下照射20分钟。照射期间，用吖啶橙处理的大鼠肿瘤发出明亮荧光。照射期间未检测到明显的温度升高。氩激光照射显著延长了用吖啶橙处理的大鼠的存活时间。组织学检查显示，联合治疗的大鼠出现了完全或部分肿瘤坏死，周围黏膜未受影响。相差显微镜和电子显微镜检查表明，细胞毒性是由细胞膜、核膜和溶酶体膜的变化介导的。单独使用染料或激光均无任何效果。
