Tatsuta M, Iishi H, Yamamura H, Yamamoto R, Okuda S
Department of Gastrointestinal Oncology, Center for Adult Diseases, Osaka, Japan.
Oncology. 1988;45(1):35-40. doi: 10.1159/000226527.
The photodynamic inactivations of Walker carcinosarcoma 256 stomach tumors by the concomitant use of acridine orange (AO) and argon laser, and by the combined use of hematoporphyrin derivatives (HPD) and dye laser were compared. Wistar rats bearing stomach tumors of 4-6 mm in diameter 5-10 days after their implantation were injected intraperitoneally with 40 mg/kg body weight of AO or HPD, and 24 h later their stomach tumors were exposed to argon laser at 488 nm or dye laser at 630 nm, respectively, at an intensity of 15 mW/cm2 for 20 min. Temperature rise was less than 3 degrees C during irradiation. Seven days after irradiation, complete or partial necrosis with sparing of the surrounding mucosa was seen histologically in all rats treated by the two therapy methods. Phase contrast and electron microscopy showed nuclear pyknosis and damage to the inner layer of the nuclear envelope in tumor cells treated with AO and argon laser, while cytotoxicity involved damage to the outer layer of the nuclear envelope and intracytoplasmic organelles in tumor cells treated with HPD and dye laser. This difference between AO and HPD is considered to be a difference in their intracellular localization in tumor cells.
比较了吖啶橙（AO）与氩激光联合使用以及血卟啉衍生物（HPD）与染料激光联合使用对Walker 256胃癌肉瘤的光动力灭活效果。将植入后5至10天、胃部长有直径4至6毫米肿瘤的Wistar大鼠腹腔注射40毫克/千克体重的AO或HPD，24小时后，分别用强度为15毫瓦/平方厘米的488纳米氩激光或630纳米染料激光照射其胃部肿瘤20分钟。照射期间温度升高小于3摄氏度。照射7天后，在两种治疗方法处理的所有大鼠中，组织学上均可见完全或部分坏死，周围黏膜未受影响。相差显微镜和电子显微镜显示，AO与氩激光处理的肿瘤细胞出现核固缩以及核膜内层受损，而HPD与染料激光处理的肿瘤细胞的细胞毒性涉及核膜外层和胞浆内细胞器受损。AO和HPD之间的这种差异被认为是它们在肿瘤细胞内定位的差异。
