Ashton I K, Soul J H
Calcif Tissue Int. 1984 Sep;36(5):576-9. doi: 10.1007/BF02405369.
Multiplication-stimulating activity (125I-MSA) has been shown to bind to isolated rabbit chondrocytes, the binding being dependent on time, temperature, and cell density. Nonspecific binding was approximately 15%. Unlabelled MSA at 100 ng/ml inhibited 125I-MSA binding by 50%. Porcine insulin (0.5-10 micrograms/ml) did not compete with MSA but resulted in a 10-15% increase in 125I-MSA binding. The data suggest that normal chondrocytes carry IGF2-type receptors as well as the IGF1 type previously described.
增殖刺激活性（125I-MSA）已被证明可与分离的兔软骨细胞结合，这种结合依赖于时间、温度和细胞密度。非特异性结合约为15%。100 ng/ml的未标记MSA可抑制50%的125I-MSA结合。0.5 - 10微克/毫升的猪胰岛素不与MSA竞争，但会使125I-MSA结合增加10 - 15%。数据表明，正常软骨细胞携带IGF2型受体以及先前描述的IGF1型受体。
