Fitzmaurice W P, Benjamin R C, Huang P C, Scocca J J
Gene. 1984 Nov;31(1-3):187-96. doi: 10.1016/0378-1119(84)90209-9.
The 32.4-kb genome of the Haemophilus influenzae bacteriophage HP1c1 contains at least twelve sites, each conferring high affinity for the DNA uptake system of transformable H. influenzae Rd. Five of these high-affinity sites have been located and their nucleotide sequences determined. Three sites contained a contiguous 9-bp sequence identical to the first nine residues of the 11-bp site previously identified as conferring high affinity for the H. influenzae transformation receptor to DNA fragments. The remaining two sites contained complete 11-bp sequences. In contrast, an HP1c1 restriction fragment containing a sequence identical to the final nine residues of the 11-bp uptake site exhibits only a low affinity for the DNA uptake system. An 8-bp sequence consisting of the first eight residues of the 11-bp site was 1% as active as the longer, high-affinity sites. Thus the first 9-bp of the 11-bp site are sufficient to direct high-affinity uptake, while the first 8-bp or the distal 9-bp are not. These results provide an initial assessment of the relative contributions of the individual residues constituting the 11-bp site to the apparent affinity of DNA fragments for the receptor of Haemophilus transformation.
流感嗜血杆菌噬菌体HP1c1的32.4千碱基基因组至少包含12个位点，每个位点对可转化的流感嗜血杆菌Rd的DNA摄取系统具有高亲和力。其中5个高亲和力位点已被定位并确定了其核苷酸序列。3个位点包含一个连续的9碱基序列，与先前确定的对流感嗜血杆菌转化受体对DNA片段具有高亲和力的11碱基位点的前9个残基相同。其余2个位点包含完整的11碱基序列。相比之下，一个含有与11碱基摄取位点的最后9个残基相同序列的HP1c1限制性片段对DNA摄取系统仅表现出低亲和力。一个由11碱基位点的前8个残基组成的8碱基序列的活性仅为较长的高亲和力位点的1%。因此，11碱基位点的前9个碱基足以指导高亲和力摄取，而前8个碱基或末端9个碱基则不行。这些结果初步评估了构成11碱基位点的各个残基对DNA片段与流感嗜血杆菌转化受体的表观亲和力的相对贡献。
