Restriction map and location of mutations on the genome of bacteriophage Hp1c1 of Haemophilus influenzae Rd.

A physical map of the 32.4-kb chromosome of the Haemophilus influenzae bacteriophage Hp1c1 has been constructed, using the cleavage sites of eight restriction endonucleases. Two temperature-sensitive mutations have also been localized on the phage chromosome. The phage DNA exhibited an affinity for the specific DNA receptor of Haemophilus transformation approx. 1.5-fold higher than that obtained with bulk chromosomal DNA of H. influenzae.