Magnani M, Mantovani P, Pepeu G
Neuropharmacology. 1984 Nov;23(11):1305-9. doi: 10.1016/0028-3908(84)90050-9.
The effect of cholecystokinin octapeptide (CCK-8) and its analogue, ceruletide on release of acetylcholine (ACh) from the cerebral cortex was investigated in urethane-anaesthetized and in unanaesthetized rats. Cholecystokinin octapeptide and ceruletide markedly stimulated output of ACh at doses of 1.5 and 5.0 micrograms/kg (i.p.), respectively. This effect was prevented by proglumide (160 mg/kg i.p.), a specific cholecystokinin receptor antagonist. At doses of 10 micrograms/kg (i.p.) and more, both CCK-8 and ceruletide decreased output of ACh from the cerebral cortex. The decrease was prevented by naloxone (1 mg/kg, s.c.), and replaced by a short-lasting increase. Cholecystokinin octapeptide and ceruletide appear therefore to affect the activity of cortical cholinergic fibres by acting upon both specific and opiate receptors. The interaction between CCK-8 and ceruletide, and opiate receptors either direct or through the release of endogenous opiates, was also demonstrated by the antagonism between ceruletide (1, 5 and 10 micrograms/kg, i.p.) and analgesia induced by morphine (5 mg/kg, s.c.), evaluated by the tail-flick test in the rat.
在乌拉坦麻醉和未麻醉的大鼠中，研究了八肽胆囊收缩素（CCK - 8）及其类似物蛙皮素对大脑皮层乙酰胆碱（ACh）释放的影响。八肽胆囊收缩素和蛙皮素分别以1.5和5.0微克/千克（腹腔注射）的剂量显著刺激ACh的释放。这种作用被特异性胆囊收缩素受体拮抗剂丙谷胺（160毫克/千克腹腔注射）所阻断。当剂量为10微克/千克（腹腔注射）及以上时，CCK - 8和蛙皮素均降低大脑皮层ACh的释放。这种降低被纳洛酮（1毫克/千克，皮下注射）所阻断，并被短暂的增加所取代。因此，八肽胆囊收缩素和蛙皮素似乎通过作用于特异性受体和阿片受体来影响皮层胆碱能纤维的活性。通过大鼠甩尾试验评估，蛙皮素（1、5和10微克/千克，腹腔注射）与吗啡（5毫克/千克，皮下注射）诱导的镇痛之间的拮抗作用也证明了CCK - 8和蛙皮素与阿片受体之间的相互作用，这种相互作用可能是直接的，也可能是通过内源性阿片类物质的释放介导的。
