Lundberg C, Arfors K E
Inflammation. 1983 Sep;7(3):247-55. doi: 10.1007/BF00917262.
Two methods for quantitating polymorphonuclear leukocyte (PMN) accumulation in inflammatory skin lesions were studied. The lesions were produced in rats by intradermal injections of different dilutions of zymosan-activated plasma (ZAP). PMN accumulation in the skin lesions was estimated by determination of (1) homologous 51Cr-labeled PMNs and (2) activity of myeloperoxidase (MPO) in the tissue sample. 125I-labeled human serum albumin was used for measurement of albumin extravasation. The [51Cr]PMN content and MPO activity in the skin lesions were both proportional to the concentration of ZAP injected. The correlation coefficient (r) between the two methods of measuring PMN accumulation in the inflammatory skin lesions was calculated to be 0.81 +/- 0.13 (mean +/- SD, N = 8). The proportionality of the PMN accumulation to the different dilutions of injected ZAP, as measured both by [51Cr]PMN and by MPO activity, and the correlation of the two methods to each other, suggest that these two methods are reliable for measuring PMN accumulation in vivo. The inflammatory reaction also included albumin extravasation, which reached a relatively high level already at the lowest concentration of injected ZAP and did not seem to parallel PMN accumulation.
研究了两种定量炎症性皮肤病变中多形核白细胞（PMN）积聚的方法。通过皮内注射不同稀释度的酵母聚糖激活血浆（ZAP）在大鼠身上产生病变。通过测定（1）同源51Cr标记的PMN和（2）组织样本中髓过氧化物酶（MPO）的活性来估计皮肤病变中PMN的积聚。使用125I标记的人血清白蛋白测量白蛋白外渗。皮肤病变中的[51Cr]PMN含量和MPO活性均与注射的ZAP浓度成正比。计算出两种测量炎症性皮肤病变中PMN积聚方法之间的相关系数（r）为0.81±0.13（平均值±标准差，N = 8）。通过[51Cr]PMN和MPO活性测量，PMN积聚与注射的不同稀释度ZAP的比例关系，以及两种方法之间的相关性，表明这两种方法对于测量体内PMN积聚是可靠的。炎症反应还包括白蛋白外渗，其在注射的ZAP最低浓度时就已达到相对较高水平，并且似乎与PMN积聚不平行。
