Issekutz A C, Movat H Z
Lab Invest. 1980 Mar;42(3):310-7.
This report describes the in vivo quantitation of neutrophil accumulation at inflammatory sites in rabbits by employing 51Cr-labeled autologous rabbit blood neutrophils. These labeled neutrophils circulated with a half of 3.2 to 3.8 hours. They were found to localize with great specificity at skin sites injected intradermally with zymosan-activated plasma, synthetic chemotactic peptides (e.g., N-formyl methionyl leucyl phenylalanine), Escherichia coli-derived chemotactic factors, or whole E. coli. Contrary to reports utilizing in vitro assays, under in vivo conditions the synthetic chemotactic peptides caused significantly less neutrophil accululation than zymosan-aulation by high concentrations of N-formyl methionyl leucyl phenylalanine was observed. Kinetic studies of the accumulation of labeled leukocytes in the skin in response to intradermal injection of formalin-killed E. coli were performed. Nearly all of the leukocytes accumulated during the first 4 hours after E. coli injection with a peak rate of accumulation between 2 and 3 hours. Essentially no additional localization of leukocytes occurred at lesions which were 6, 8, or 24 hours old. These results demonstrate that 51Cr-labeled rabbit blood neutrophils can be utilized to quantitate the degree of neutrophil accumulation in inflammatory reactions as well as to study the hour by hour kinetics of this accumulation.
本报告描述了通过使用51Cr标记的自体兔血中性粒细胞对兔炎症部位中性粒细胞聚集进行体内定量分析。这些标记的中性粒细胞的半衰期为3.2至3.8小时。研究发现，它们能高度特异性地定位于皮内注射酵母聚糖激活血浆、合成趋化肽（如N-甲酰甲硫氨酰亮氨酰苯丙氨酸）、大肠杆菌衍生趋化因子或完整大肠杆菌的皮肤部位。与利用体外试验的报告相反，在体内条件下，合成趋化肽引起的中性粒细胞聚集明显少于酵母聚糖激活血浆，且观察到高浓度的N-甲酰甲硫氨酰亮氨酰苯丙氨酸会导致这种情况。对皮内注射福尔马林灭活大肠杆菌后皮肤中标记白细胞聚集进行了动力学研究。几乎所有白细胞在注射大肠杆菌后的前4小时内聚集，聚集峰值出现在2至 3小时之间。在6、8或24小时龄的病变部位基本上没有发生额外的白细胞定位。这些结果表明，51Cr标记的兔血中性粒细胞可用于定量炎症反应中中性粒细胞聚集的程度，以及研究这种聚集的逐时动力学。
