Schlaghecke R, Kley H K, Krüskemper H L
Steroids. 1984 Jul;44(1):23-33. doi: 10.1016/s0039-128x(84)80013-6.
A radioimmunoassay (RIA) method is described for the determination of 4-androstene-3, 11, 17-trione (11-oxo-androstenedione) in human plasma. 4-androstene-3, 11, 17-trione 3-(0-carboxymethyl) oxime-bovine serum albumin conjugate was used to generate highly specific antiserum in rabbits. Cross reactivities of several other steroids with the antiserum were less than 4%. [1,2-3H] 4-androstene-3, 11, 17-trione was synthesized from [1,2-3H] 17 alpha, 21-dihydroxy-4-pregnene-3, 11, 20-trione. The intra- and interassay variation was 7.3% and 9.8%, respectively. The mean serum 4-androstene-3, 11, 17-trione level for healthy young subjects was 2.37 +/- 0.56 nM (X +/- SD) in males and 3.16 +/- 0.43 nM in females at 8 a.m. During the night, there was a marked decrease in serum level, giving at 11 p.m. 0.87 +/- 0.33 and 1.15 +/- 0.52 nM, respectively. During ACTH stimulation tests, 4-androstene-3, 11, 17-trione increased from 1.81 +/- 0.58 to 2.32 +/- 0.69 nM, while in dexamethasone suppression tests a decrease from 3.20 +/- 0.03 nM was seen. In contrast, HCG administration on 3 consecutive days did not influence plasma concentrations of 4-androstene-3, 11, 17-trione.
本文描述了一种用于测定人血浆中4-雄烯-3,11,17-三酮（11-氧代-雄烯二酮）的放射免疫分析（RIA）方法。使用4-雄烯-3,11,17-三酮3-(O-羧甲基)肟-牛血清白蛋白偶联物在兔体内产生高度特异性抗血清。几种其他类固醇与该抗血清的交叉反应率低于4%。[1,2-³H]4-雄烯-3,11,17-三酮由[1,2-³H]17α,21-二羟基-4-孕烯-3,11,20-三酮合成。批内和批间变异分别为7.3%和9.8%。健康年轻男性受试者上午8点时血清4-雄烯-3,11,17-三酮的平均水平为2.37±0.56 nM（X±SD），女性为3.16±0.43 nM。夜间血清水平显著下降，晚上11点时分别为0.87±0.33和1.15±0.52 nM。在促肾上腺皮质激素（ACTH）刺激试验期间，4-雄烯-3,11,17-三酮从1.81±0.58 nM增加到2.32±0.69 nM，而在地塞米松抑制试验中则从3.20±0.03 nM下降。相比之下，连续3天给予人绒毛膜促性腺激素（HCG）并未影响4-雄烯-3,11,17-三酮的血浆浓度。
