一种改进的用于检测4-雄烯-3,17-二酮的放射免疫分析法。

An improved radioimmunoassay for 4-androstene-3,17-dione.

作者信息

Parker L N, Grover P K, Odell W D

出版信息

Steroids. 1977 Jun;29(6):715-24. doi: 10.1016/0039-128x(77)90116-7.

Abstract

A radioimmunoassay using an antiserum produced against 6beta-hydroxy-4-androstene-3,17-dione-6-succinyl-BSA conjugate is described which permits the rapid determination of 4-androstene-3,17-dione in multiple serum samples that are purified by column chromatography on neutral alumina. Steroids which reacted significantly with the antiserum were found to be 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, and 6beta-hydroxy-4-androstene-3,17-dione. After column chromatography on alumina, however, the only significantly cross-reacting steroids were the 5alpha and 5beta-androstane-3,17-diones, while cross-reactivity from other steroids was reduced to less than 1%.

摘要

本文描述了一种放射免疫测定法，该方法使用针对6β-羟基-4-雄烯-3,17-二酮-6-琥珀酰-BSA偶联物产生的抗血清，可快速测定通过中性氧化铝柱色谱纯化的多个血清样品中的4-雄烯-3,17-二酮。发现与抗血清有显著反应的类固醇为5α-雄烷-3,17-二酮、5β-雄烷-3,17-二酮和6β-羟基-4-雄烯-3,17-二酮。然而，在氧化铝柱色谱后，唯一有显著交叉反应的类固醇是5α和5β-雄烷-3,17-二酮，而其他类固醇的交叉反应性降低到不到1%。