Obreshkov K, Dilovski M
Vet Med Nauki. 1977;14(10):9-13.
The plaque technique is applied to the type differentiation of the Newcastle disease viruses, 0.5 per cent agarose being used as a nutritive overlayer. The epizootic Newcastle disease strains are characterized by a heterogenic plaque population in which the large plaques are prevalent, averaging 3--4 mm, with an irregular round form, an unclearly delineated diffuse edge, and a red center. The vaccinal strains (B1 and K) yield a homogeneous small-plaque population, the plaques themselves being 1--2 mm large, with a regular round shape, a well delineated edge, and a bright center.
噬斑技术用于新城疫病毒的分型,用0.5%的琼脂糖作为营养覆盖层。流行的新城疫病毒株的特征是噬斑群体具有异质性,其中大噬斑占优势,平均直径为3-4毫米,呈不规则圆形,边缘弥散不清晰,中心为红色。疫苗株(B1和K)产生均匀的小噬斑群体,噬斑本身直径为1-2毫米,呈规则圆形,边缘清晰,中心明亮。