Blejer J L, Lerman G D, Nejamkis M R
Cátedra de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UBA, Argentina.
Rev Argent Microbiol. 1984;16(1):11-8.
The 2-day-old rat is known to resist intracerebral infection with the XJ prototype strain of Junin virus, but 95-100% mortality results when infected with the attenuated XJC13 strain. When this animal was inoculated by intraperitoneal route, behaviour was diametrically opposite: the XJ strain proved lethal, while de XJC13 led to low mortality. Studies on mortality, virus titer in different organs, and anti-viral humoral response in 2-day-old rats infected with Junin virus strains were carried out in order to use this system as a new attenuation marker. Mortality rates recorded for rats inoculated with either strain, were markedly different, being 84% in the XJ-infected group and barely reaching 17% in the XJC13 group. Brain viral titers were higher in the former group than the latter (10(5.26) PFU/ml vs. 10(3) PFU/ml at day 17 pi). For this reason, viral replication may be used as a virulence marker in this experimental model. Antibody levels were also higher in the XJ group most likely due to greater viral replication. The above findings support the use of the 2-day-old rat as a biologic attenuation marker since susceptibility to infection is strain-dependent.
已知2日龄大鼠能抵抗朱宁病毒XJ原型株的脑内感染，但感染减毒的XJC13株时死亡率达95 - 100%。当通过腹腔途径接种该动物时，情况则完全相反：XJ株具有致死性，而XJC13株导致的死亡率较低。为了将该系统用作新的减毒标记物，对感染朱宁病毒株的2日龄大鼠的死亡率、不同器官中的病毒滴度以及抗病毒体液反应进行了研究。接种任一毒株的大鼠的死亡率记录明显不同，XJ感染组为84%，而XJC13组仅为17%。前一组的脑病毒滴度高于后一组（感染后17天分别为10(5.26) PFU/ml和10(3) PFU/ml）。因此，在该实验模型中，病毒复制可作为毒力标记物。XJ组的抗体水平也更高，很可能是由于病毒复制更多。上述发现支持将2日龄大鼠用作生物学减毒标记物，因为对感染的易感性取决于毒株。
