Dobosch D
Centers for Disease Control, Atlanta, Georgia.
Rev Argent Microbiol. 1983;15(3):163-8.
The staphylococcal coagglutination technique has been used in recent years for detecting various bacterial antigens and toxins. It is a simple and rapid technique which requires no specialized equipment. This report describes a staphylococcal coagglutination procedure for assaying C. perfringens enterotoxin. Its sensitivity and specificity were studied with the aid of various enterotoxin preparations and culture filtrates of C. perfringens and other Clostridium species. This assay detects between 1.9 and 3.8 micrograms of enterotoxin per ml. Only the enterotoxin preparations and the C. perfringens culture fluids were positive.
近年来，葡萄球菌协同凝集技术已被用于检测各种细菌抗原和毒素。这是一种简单快速的技术，不需要专门的设备。本报告描述了一种用于检测产气荚膜梭菌肠毒素的葡萄球菌协同凝集程序。借助各种肠毒素制剂以及产气荚膜梭菌和其他梭菌属的培养滤液，对其敏感性和特异性进行了研究。该检测方法可检测出每毫升1.9至3.8微克的肠毒素。只有肠毒素制剂和产气荚膜梭菌培养液呈阳性。
