Hostacká A, Majtán V
Cesk Epidemiol Mikrobiol Imunol. 1989 Mar;38(2):89-95.
The authors examined 39 strains of E. coli isolated from patients with the clinical diagnosis of diarrhoea for the presence of thermolabile enterotoxin (LT-I). For assessment of LT-I adaptation of the staphylococcal coagglutination technique was used. Suspension of a strain of staphylococcus aureus Cowan I, MAU, 55/64 was sensitized with a specific antibody against LT-I (not diluted and diluted). 50 ng purified enterotoxin created with the thus sensitized staphylococcal suspension a still reproducible coagglutination. The above test revealed that none of the tested strains of E. coli produced the thermolabile LT-I enterotoxin.
作者检测了从临床诊断为腹泻的患者中分离出的39株大肠杆菌，以确定是否存在不耐热肠毒素（LT-I）。为评估LT-I，采用了葡萄球菌协同凝集技术。用抗LT-I的特异性抗体（未稀释和稀释的）致敏金黄色葡萄球菌Cowan I、MAU、55/64菌株的悬液。50纳克纯化肠毒素与如此致敏的葡萄球菌悬液产生了仍可重复的协同凝集。上述试验表明，所检测的大肠杆菌菌株均未产生不耐热LT-I肠毒素。
