Midha K K, Cooper J K, McGilveray I J, Butterfield A G, Hubbard J W
College of Pharmacy, University of Saskatchewan, Saskatoon, Canada.
J Pharm Sci. 1981 Sep;70(9):1043-6. doi: 10.1002/jps.2600700920.
A specific and sensitive high-performance liquid chromatographic (HPLC) method for the quantitative determination of plasma chlorpromazine concentrations is described. The procedure is capable of determining 1 ng of chlorpromazine/ml and is adequate for following plasma concentration-time profiles after 7-mg single intravenous doses. After a simple organic extraction of the drug and an internal standard (mesoridazine) from plasma, the organic layer was transferred to a vial and evaporated to dryness at 55 degrees under nitrogen. The residue was dissolved in 200 microliters of HPLC grade acetonitrile. Aliquots (70-100 microliters) were chromatographed, and the drug was quantitated in the range of 1-15 ng/ml of plasma using a fixed-wavelength UV detector. Plasma concentrations determined by the method were compared with those obtained by a previously reported radioimmunoassay specific for chlorpromazine and N-desmethylchlorpromazine. The two methods agreed favorably with a correlation coefficient of 0.993 and a slope of 0.994.
本文描述了一种用于定量测定血浆中氯丙嗪浓度的特异性和灵敏的高效液相色谱（HPLC）方法。该方法能够测定1纳克氯丙嗪/毫升，适用于跟踪单次静脉注射7毫克后血浆浓度-时间曲线。从血浆中对药物和内标（美索达嗪）进行简单的有机萃取后，将有机层转移至小瓶中，并在55℃下氮气吹干。残渣溶于200微升HPLC级乙腈中。取70 - 100微升等分试样进行色谱分析，使用固定波长紫外检测器在1 - 15纳克/毫升血浆范围内对药物进行定量。将该方法测定的血浆浓度与先前报道的针对氯丙嗪和N -去甲基氯丙嗪的放射免疫分析法所得结果进行比较。两种方法结果良好一致，相关系数为0.993，斜率为0.994。
