McKay G, Hall K, Cooper J K, Hawes E M, Midha K K
J Chromatogr. 1982 Nov 12;232(2):275-82.
A gas chromatographic--mass spectrometric assay using selected ion monitoring is compared with a high-performance liquid chromatographic assay using an electrochemical detector for single-dose studies of the psychotherapeutic phenothiazine drug chlorpromazine. Measurements were made after extraction of chlorpromazine and the internal standard, prochlorperazine, from basified plasma with an isopropanol--pentane solvent mixture. Following evaporation of the organic solvents the residue was reconstituted in a small volume of methanol and subjected to gas chromatographic--mass spectrometric selected ion detection. The residual sample was then evaporated and made up in a larger volume of acetonitrile and analyzed by high-performance liquid chromatography using an electrochemical detector. These specific methods display excellent correlation for plasma concentration determinations in the range of 0.25-10 ng ml-1 and will allow for the study of the pharmacokinetics of chlorpromazine following single low doses of the drug.
采用选择离子监测的气相色谱-质谱分析法与使用电化学检测器的高效液相色谱分析法进行比较，用于对精神治疗性吩噻嗪类药物氯丙嗪进行单剂量研究。在用异丙醇-戊烷溶剂混合物从碱化血浆中提取氯丙嗪和内标物奋乃静后进行测量。有机溶剂蒸发后，残留物用少量甲醇复溶，并进行气相色谱-质谱选择离子检测。然后将剩余样品蒸发，并在较大体积的乙腈中复溶，使用电化学检测器通过高效液相色谱法进行分析。这些特定方法在0.25 - 10 ng/ml范围内的血浆浓度测定中显示出极好的相关性，并将有助于研究单次低剂量药物后氯丙嗪的药代动力学。
