Schiff P B, Horwitz S B
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1561-5. doi: 10.1073/pnas.77.3.1561.
Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90% of the cells treated with 10 micro M taxol for 22 hr at 37 degrees C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4 degrees C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37 degrees C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.
紫杉醇是一种有效的人类宫颈癌细胞系HeLa和小鼠成纤维细胞复制抑制剂，可使细胞阻滞于细胞周期的G2期和M期，并使细胞质微管稳定。通过透射电子显微镜和间接免疫荧光显微镜观察了经紫杉醇处理的细胞的细胞质微管。在37℃下用10微摩尔/升紫杉醇处理细胞22小时，超过90%的细胞除了有细胞质微管外，还显示出从一个或多个共同位点放射状发出的微管束。未经处理的细胞在4℃下冷藏16小时后微管消失，而在37℃下先用紫杉醇处理22小时的细胞在冷藏后仍继续显示其微管和微管束。紫杉醇抑制成纤维细胞的迁移行为，但这些细胞产生诸如片状伪足和丝状伪足等可移动表面突起的能力并未丧失。
