Chang T M, Chey W Y
Dig Dis Sci. 1980 Jul;25(7):529-52. doi: 10.1007/BF01315215.
The radioimmunoassay methods of secretin are reviewed with respect to production of antibody, preparation of radioactive tracers, and effect of plasma interference. The major difference in the secretin assay methods resides in handling plasma interference. Thus the assay sensitivity decreased markedly when the assay was conducted by diluting plasma samples. When the assay was conducted by compensating for plasma interference with homologous hormone-free plasma, the effect of plasma interference was greatly reduced, leading to a more sensitive assay. However, this method probably can not obtain consistent results with plasma samples collected under various experimental conditions. The method is still subject to considerable desensitization and assay variation. On the other hand, the elimination of plasma interference before assay results in the most sensitive secretin assays capable of detecting consistently a significant postprandial rise in plasma secretin level. It is concluded that a sensitive, validated secretin radioimmunoassay should be one that is capable of detecting increments of plasma secretin in response to doses of intraduodenal acid at 0.055 mEq/min or lower and intravenous administration of exogenous secretin at 0.03 CU/kg/hr with concomitant stimulation of pancreatic bicarbonate and water secretion. With a sensitive and accurate radioimmunoassay for secretin, it is now possible to further investigate the physiology and pathophysiology of secretin.
本文就促胰液素的放射免疫测定方法，从抗体的产生、放射性示踪剂的制备以及血浆干扰的影响等方面进行了综述。促胰液素测定方法的主要差异在于对血浆干扰的处理。因此，当通过稀释血浆样本进行测定时，测定灵敏度会显著降低。当通过用同源无激素血浆补偿血浆干扰来进行测定时，血浆干扰的影响会大大降低，从而使测定更灵敏。然而，对于在各种实验条件下采集的血浆样本，这种方法可能无法获得一致的结果。该方法仍然存在相当程度的脱敏和测定差异。另一方面，在测定前消除血浆干扰可得到最灵敏的促胰液素测定方法，能够始终检测到餐后血浆促胰液素水平的显著升高。结论是，一种灵敏、经过验证的促胰液素放射免疫测定方法应该能够检测到十二指肠内酸以0.055毫当量/分钟或更低的速率给药以及静脉注射0.03单位/千克/小时的外源性促胰液素时血浆促胰液素的增加，并伴随胰腺碳酸氢盐和水分泌的刺激。有了灵敏且准确的促胰液素放射免疫测定方法，现在就有可能进一步研究促胰液素的生理学和病理生理学。
