Stagni N, de Bernard B, Liut G F, Vittur F, Zanetti M
Connect Tissue Res. 1980;7(3):121-5. doi: 10.3109/03008208009152103.
The discovery in calcifying cartilage of a glycoprotein, endowed with high calcium affinity and alkaline phosphatase activity, has prompted the investigation of the presence of this compound in other calcified tissues. From medullary bone, a tissue which is highly mineralized under estrogen stimulus, a glycoprotein has been extracted which had the properties described. Besides the high calcium affinity (KD = 10(-7)M), this protein shows phosphatase activity and rate of hydrolysis of ATP, GTP and pyrophosphate was measured. Analysis of the chemical composition of the matrix of the medullary bone indicates that proteoglycans are present in large amounts. The calcium binding glycoprotein appears to be a compound present in different calcified tissues.
在钙化软骨中发现了一种具有高钙亲和力和碱性磷酸酶活性的糖蛋白，这促使人们研究该化合物在其他钙化组织中的存在情况。从髓质骨（一种在雌激素刺激下高度矿化的组织）中提取出了一种具有上述特性的糖蛋白。除了高钙亲和力（解离常数KD = 10^(-7)M）外，这种蛋白质还具有磷酸酶活性，并测定了其对ATP、GTP和焦磷酸的水解速率。对髓质骨基质化学成分的分析表明，蛋白聚糖大量存在。钙结合糖蛋白似乎是一种存在于不同钙化组织中的化合物。
