筛选可阻止F质粒功能表达的大肠杆菌K-12染色体突变体。
Selection of Escherichia coli K-12 chromosomal mutants that prevent expression of F-plasmid functions.
作者信息
Silverman P, Nat K, McEwen J, Birchman R
出版信息
J Bacteriol. 1980 Sep;143(3):1519-23. doi: 10.1128/jb.143.3.1519-1523.1980.
Abstract
Chromosomal mutants of Escherichia coli deficient in the expression of F-plasmid functions were selected by mutagenizing F- cells, introducing an F' plasmid into the mutagenized cells by conjugation, and identifying transconjugants resistant to the donor-specific bacteriophage Q beta by a simple spray test. All but 1 of 25 mutants were defective in an extracellular stage of Q beta infection, suggesting that they fail to elaborate F-pili. At least six of these were also deficient as deoxyribonucleic acid donors. More than half of the mutants appear to be altered in peviously undetected chromosomal genes required for the expression of F-related cellular functions.
摘要
通过诱变F-细胞，通过接合将F'质粒导入诱变细胞，并通过简单的喷雾试验鉴定对供体特异性噬菌体Qβ具有抗性的转接合子，从而筛选出缺乏F质粒功能表达的大肠杆菌染色体突变体。25个突变体中除1个外，其余均在Qβ感染的细胞外阶段存在缺陷，这表明它们无法形成F菌毛。其中至少6个作为脱氧核糖核酸供体也存在缺陷。超过一半的突变体似乎在表达F相关细胞功能所需的先前未检测到的染色体基因中发生了改变。
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